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Vibrant scientific diagram illustrating molecular purification processes, with streams of light and bubbles representing Protein A, PH Buffers, Viral Retention, Filtration, Chromatography, and Cation Exchange.

peux tu me generer une image qui decrit ça stp .2.2. Rôle et périmètre de l'équipe DSP (Downstream Processing) : - L'équipe DSP assure la purification, la sécurisation virale et la reformulation finale de l'anticorps. Elle utilise divers tampons (de lavage, d'élution, d'inactivation ou de formulation) pour réguler le pH et garantir la stabilité de la molécule au fil des étapes. • Capture d'affinité (Protéine A) : Isoler l'anticorps monoclonal du milieu de culture. Cette étape initiale contribue déjà activement à la clairance virale par élimination physique d'une grande partie des particules virales. • Inactivation virale & Filtration profonde : Traitement à bas pH pour inactiver spécifiquement les virus enveloppés, suivi d'une filtration profonde de clarification intermédiaire. • Filtration virale & Polissages chromatographiques : o Filtration virale : Rétention physique dédiée des particules virales o Chromatographie multimodale (MMC). o Chromatographie d'échange de cations (CEX) en mode négatif (flow-through) : Élimination des impuretés résiduelles comme les agrégats o Filtration tangentielle (TFF) & Reformulation : Diafiltration pour changement de tampon et ajustement de la concentration protéique cible. o Conditionnement intermédiaire : Une fois reformulé, l'anticorps sous forme de produit intermédiaire (non prêt à l'usage) est transféré vers un site tiers chargé d'assurer sa mise en forme pharmaceutique finale Ver mais

#science #biotechnology

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